Construct: shRNA TRCN0000276334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025114.3-543s21c1
DNA Barcode:
GAGCTGGCTTTGGAAGAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CEP290 (80184)
Gene Description:
centrosomal protein 290
Transcript:
RefSeq NM_025114.3 (NON-CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTGGCTTTGGAAGAAGTA
Hairpin Sequence:
5'-CCGG-GAGCTGGCTTTGGAAGAAGTA-CTCGAG-TACTTCTTCCAAAGCCAGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTGGCTTTGGAAGAAGTACTCGAGTACTTCTTCCAAAGCCAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTGGCTTTGGAAGAAGTACTCGAGTACTTCTTCCAAAGCCAGCTC-3'

Other clones with same target sequence:

  1. TRCN0000142313