Construct: shRNA TRCN0000276538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004093.2-280s21c1
DNA Barcode:
AGCAGACAGATGCACTATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EFNB2 (1948)
Gene Description:
ephrin B2
Transcript:
RefSeq NM_004093.2 (NON-CURRENT)
Match location:
Position 280 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGACAGATGCACTATTAA
Hairpin Sequence:
5'-CCGG-AGCAGACAGATGCACTATTAA-CTCGAG-TTAATAGTGCATCTGTCTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGACAGATGCACTATTAACTCGAGTTAATAGTGCATCTGTCTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGACAGATGCACTATTAACTCGAGTTAATAGTGCATCTGTCTGCT-3'

Other clones with same target sequence:

(none)