Construct: shRNA TRCN0000276564

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004927.2-1910s21c1
DNA Barcode:
CCTGGAGATTCCATTCCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRPL49 (740)
Gene Description:
mitochondrial ribosomal protein L49
Transcript:
RefSeq NM_004927.2 (NON-CURRENT)
Match location:
Position 1910 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276564)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAGATTCCATTCCATTT
Hairpin Sequence:
5'-CCGG-CCTGGAGATTCCATTCCATTT-CTCGAG-AAATGGAATGGAATCTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAGATTCCATTCCATTTCTCGAGAAATGGAATGGAATCTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAGATTCCATTCCATTTCTCGAGAAATGGAATGGAATCTCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000178727