Construct: shRNA TRCN0000276641

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133945.1-900s21c1
DNA Barcode:
GCTCTGGAGTATCTCCATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vrk3 (101568)
Gene Description:
vaccinia related kinase 3
Transcript:
RefSeq NM_133945.1 (CURRENT)
Match location:
Position 900 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276641)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGGAGTATCTCCATGAA
Hairpin Sequence:
5'-CCGG-GCTCTGGAGTATCTCCATGAA-CTCGAG-TTCATGGAGATACTCCAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGGAGTATCTCCATGAACTCGAGTTCATGGAGATACTCCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGGAGTATCTCCATGAACTCGAGTTCATGGAGATACTCCAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000023786