Construct: shRNA TRCN0000276804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021448.2-2596s21c1
DNA Barcode:
CTTTACCTTCGTGATTGATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Elp2 (58523)
Gene Description:
elongator acetyltransferase complex subunit 2
Transcript:
RefSeq NM_021448.2 (CURRENT)
Match location:
Position 2596 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTACCTTCGTGATTGATTG
Hairpin Sequence:
5'-CCGG-CTTTACCTTCGTGATTGATTG-CTCGAG-CAATCAATCACGAAGGTAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTACCTTCGTGATTGATTGCTCGAGCAATCAATCACGAAGGTAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTACCTTCGTGATTGATTGCTCGAGCAATCAATCACGAAGGTAAAG-3'

Other clones with same target sequence:

(none)