Construct: shRNA TRCN0000276843

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026885.2-322s21c1
DNA Barcode:
ATGGATGCTGTTCGAATCATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chmp2a (68953)
Gene Description:
charged multivesicular body protein 2A
Transcript:
RefSeq NM_026885.2 (NON-CURRENT)
Match location:
Position 322 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276843)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGATGCTGTTCGAATCATG
Hairpin Sequence:
5'-CCGG-ATGGATGCTGTTCGAATCATG-CTCGAG-CATGATTCGAACAGCATCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGATGCTGTTCGAATCATGCTCGAGCATGATTCGAACAGCATCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGATGCTGTTCGAATCATGCTCGAGCATGATTCGAACAGCATCCAT-3'

Other clones with same target sequence:

(none)