Construct: shRNA TRCN0000276844

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026885.2-457s21c1
DNA Barcode:
CAAGCCATGAAGGGTGTTACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chmp2a (68953)
Gene Description:
charged multivesicular body protein 2A
Transcript:
RefSeq NM_026885.2 (NON-CURRENT)
Match location:
Position 457 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276844)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGCCATGAAGGGTGTTACT
Hairpin Sequence:
5'-CCGG-CAAGCCATGAAGGGTGTTACT-CTCGAG-AGTAACACCCTTCATGGCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGCCATGAAGGGTGTTACTCTCGAGAGTAACACCCTTCATGGCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGCCATGAAGGGTGTTACTCTCGAGAGTAACACCCTTCATGGCTTG-3'

Other clones with same target sequence:

  1. TRCN0000182144