Construct: shRNA TRCN0000276888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019933.2-3010s21c1
DNA Barcode:
ATGTTCACTGTGCCATAATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptpn4 (19258)
Gene Description:
protein tyrosine phosphatase, non-receptor type 4
Transcript:
RefSeq NM_019933.2 (CURRENT)
Match location:
Position 3010 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTTCACTGTGCCATAATAC
Hairpin Sequence:
5'-CCGG-ATGTTCACTGTGCCATAATAC-CTCGAG-GTATTATGGCACAGTGAACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTTCACTGTGCCATAATACCTCGAGGTATTATGGCACAGTGAACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTTCACTGTGCCATAATACCTCGAGGTATTATGGCACAGTGAACAT-3'

Other clones with same target sequence:

(none)