Construct: shRNA TRCN0000276922

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133766.3-1076s21c1
DNA Barcode:
CAAGAGATTCTAGGACACCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Efr3a (76740)
Gene Description:
EFR3 homolog A
Transcript:
RefSeq NM_133766.3 (CURRENT)
Match location:
Position 1076 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276922)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAGATTCTAGGACACCTT
Hairpin Sequence:
5'-CCGG-CAAGAGATTCTAGGACACCTT-CTCGAG-AAGGTGTCCTAGAATCTCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAGATTCTAGGACACCTTCTCGAGAAGGTGTCCTAGAATCTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAGATTCTAGGACACCTTCTCGAGAAGGTGTCCTAGAATCTCTTG-3'

Other clones with same target sequence:

  1. TRCN0000193740