Construct: shRNA TRCN0000276923

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133766.3-2297s21c1
DNA Barcode:
GTATCTATTCAGGTGGATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Efr3a (76740)
Gene Description:
EFR3 homolog A
Transcript:
RefSeq NM_133766.3 (CURRENT)
Match location:
Position 2297 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276923)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATCTATTCAGGTGGATATT
Hairpin Sequence:
5'-CCGG-GTATCTATTCAGGTGGATATT-CTCGAG-AATATCCACCTGAATAGATAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATCTATTCAGGTGGATATTCTCGAGAATATCCACCTGAATAGATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATCTATTCAGGTGGATATTCTCGAGAATATCCACCTGAATAGATAC-3'

Other clones with same target sequence:

  1. TRCN0000175048