Construct: shRNA TRCN0000276958

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027093.4-672s21c1
DNA Barcode:
GCTAGCAGAAACTGAAAGGGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufaf5 (69487)
Gene Description:
NADH:ubiquinone oxidoreductase complex assembly factor 5
Transcript:
RefSeq NM_027093.4 (CURRENT)
Match location:
Position 672 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276958)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGCAGAAACTGAAAGGGA
Hairpin Sequence:
5'-CCGG-GCTAGCAGAAACTGAAAGGGA-CTCGAG-TCCCTTTCAGTTTCTGCTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGCAGAAACTGAAAGGGACTCGAGTCCCTTTCAGTTTCTGCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGCAGAAACTGAAAGGGACTCGAGTCCCTTTCAGTTTCTGCTAGC-3'

Other clones with same target sequence:

  1. TRCN0000182551