Construct: shRNA TRCN0000276962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026494.3-837s21c1
DNA Barcode:
CCAACATCCTGGAGTCAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppcs (106564)
Gene Description:
phosphopantothenoylcysteine synthetase
Transcript:
RefSeq NM_026494.3 (CURRENT)
Match location:
Position 837 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACATCCTGGAGTCAATAA
Hairpin Sequence:
5'-CCGG-CCAACATCCTGGAGTCAATAA-CTCGAG-TTATTGACTCCAGGATGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACATCCTGGAGTCAATAACTCGAGTTATTGACTCCAGGATGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACATCCTGGAGTCAATAACTCGAGTTATTGACTCCAGGATGTTGG-3'

Other clones with same target sequence:

(none)