Construct: shRNA TRCN0000276976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029157.3-1236s21c1
DNA Barcode:
GCCTGAATATCAACTACAACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sf3a3 (75062)
Gene Description:
splicing factor 3a, subunit 3
Transcript:
RefSeq NM_029157.3 (CURRENT)
Match location:
Position 1236 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAATATCAACTACAACT
Hairpin Sequence:
5'-CCGG-GCCTGAATATCAACTACAACT-CTCGAG-AGTTGTAGTTGATATTCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAATATCAACTACAACTCTCGAGAGTTGTAGTTGATATTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAATATCAACTACAACTCTCGAGAGTTGTAGTTGATATTCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000175925