Construct: shRNA TRCN0000276992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025879.2-372s21c1
DNA Barcode:
CTACTTTGTTCACCAATATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trappc13 (66975)
Gene Description:
trafficking protein particle complex 13
Transcript:
RefSeq NM_025879.2 (CURRENT)
Match location:
Position 372 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACTTTGTTCACCAATATTC
Hairpin Sequence:
5'-CCGG-CTACTTTGTTCACCAATATTC-CTCGAG-GAATATTGGTGAACAAAGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACTTTGTTCACCAATATTCCTCGAGGAATATTGGTGAACAAAGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACTTTGTTCACCAATATTCCTCGAGGAATATTGGTGAACAAAGTAG-3'

Other clones with same target sequence:

(none)