Construct: shRNA TRCN0000276994

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028860.2-1918s21c1
DNA Barcode:
GCAGGCAACAAGGCTTTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtmr3 (74302)
Gene Description:
myotubularin related protein 3
Transcript:
RefSeq NM_028860.2 (CURRENT)
Match location:
Position 1918 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276994)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGCAACAAGGCTTTCAAA
Hairpin Sequence:
5'-CCGG-GCAGGCAACAAGGCTTTCAAA-CTCGAG-TTTGAAAGCCTTGTTGCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGCAACAAGGCTTTCAAACTCGAGTTTGAAAGCCTTGTTGCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGCAACAAGGCTTTCAAACTCGAGTTTGAAAGCCTTGTTGCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000030090