Construct: shRNA TRCN0000277061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033609.2-1754s21c1
DNA Barcode:
CATGATCAACAAGATCGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Med15 (94112)
Gene Description:
mediator complex subunit 15
Transcript:
RefSeq NM_033609.2 (NON-CURRENT)
Match location:
Position 1754 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGATCAACAAGATCGACAA
Hairpin Sequence:
5'-CCGG-CATGATCAACAAGATCGACAA-CTCGAG-TTGTCGATCTTGTTGATCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGATCAACAAGATCGACAACTCGAGTTGTCGATCTTGTTGATCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGATCAACAAGATCGACAACTCGAGTTGTCGATCTTGTTGATCATG-3'

Other clones with same target sequence:

  1. TRCN0000175270