Construct: shRNA TRCN0000277076

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145528.2-1400s21c1
DNA Barcode:
GACATACCTTTCGCCATGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atg13 (51897)
Gene Description:
autophagy related 13
Transcript:
RefSeq NM_145528.2 (NON-CURRENT)
Match location:
Position 1400 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277076)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACATACCTTTCGCCATGTTT
Hairpin Sequence:
5'-CCGG-GACATACCTTTCGCCATGTTT-CTCGAG-AAACATGGCGAAAGGTATGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACATACCTTTCGCCATGTTTCTCGAGAAACATGGCGAAAGGTATGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACATACCTTTCGCCATGTTTCTCGAGAAACATGGCGAAAGGTATGTC-3'

Other clones with same target sequence:

  1. TRCN0000176029