Construct: shRNA TRCN0000277079

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032399.1-801s21c1
DNA Barcode:
CCCGTATCACTTGTGCAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr87 (84111)
Gene Description:
G protein-coupled receptor 87
Transcript:
RefSeq NM_032399.1 (NON-CURRENT)
Match location:
Position 801 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277079)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTATCACTTGTGCAGAAT
Hairpin Sequence:
5'-CCGG-CCCGTATCACTTGTGCAGAAT-CTCGAG-ATTCTGCACAAGTGATACGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTATCACTTGTGCAGAATCTCGAGATTCTGCACAAGTGATACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTATCACTTGTGCAGAATCTCGAGATTCTGCACAAGTGATACGGG-3'

Other clones with same target sequence:

  1. TRCN0000221847