Construct: shRNA TRCN0000277249

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010322.3-1022s21c1
DNA Barcode:
GATACCTACTTTGTCCCAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnpat (14712)
Gene Description:
glyceronephosphate O-acyltransferase
Transcript:
RefSeq NM_010322.3 (CURRENT)
Match location:
Position 1022 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277249)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATACCTACTTTGTCCCAATT
Hairpin Sequence:
5'-CCGG-GATACCTACTTTGTCCCAATT-CTCGAG-AATTGGGACAAAGTAGGTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATACCTACTTTGTCCCAATTCTCGAGAATTGGGACAAAGTAGGTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATACCTACTTTGTCCCAATTCTCGAGAATTGGGACAAAGTAGGTATC-3'

Other clones with same target sequence:

  1. TRCN0000174947