Construct: shRNA TRCN0000277296

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031257.2-1073s21c1
DNA Barcode:
CGCCTAACTCCATCTTGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plekha2 (83436)
Gene Description:
pleckstrin homology domain-containing, family A (phosphoinositide binding specific) member 2
Transcript:
RefSeq NM_031257.2 (NON-CURRENT)
Match location:
Position 1073 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277296)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTAACTCCATCTTGTCAA
Hairpin Sequence:
5'-CCGG-CGCCTAACTCCATCTTGTCAA-CTCGAG-TTGACAAGATGGAGTTAGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTAACTCCATCTTGTCAACTCGAGTTGACAAGATGGAGTTAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTAACTCCATCTTGTCAACTCGAGTTGACAAGATGGAGTTAGGCG-3'

Other clones with same target sequence:

  1. TRCN0000173440