Construct: shRNA TRCN0000277300

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026877.1-243s21c1
DNA Barcode:
TTGCCAACCTGCCTAACAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aspscr1 (68938)
Gene Description:
alveolar soft part sarcoma chromosome region, candidate 1 (human)
Transcript:
RefSeq NM_026877.1 (NON-CURRENT)
Match location:
Position 243 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277300)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGCCAACCTGCCTAACAATG
Hairpin Sequence:
5'-CCGG-TTGCCAACCTGCCTAACAATG-CTCGAG-CATTGTTAGGCAGGTTGGCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGCCAACCTGCCTAACAATGCTCGAGCATTGTTAGGCAGGTTGGCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGCCAACCTGCCTAACAATGCTCGAGCATTGTTAGGCAGGTTGGCAA-3'

Other clones with same target sequence:

(none)