Construct: shRNA TRCN0000277341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181411.2-2482s21c1
DNA Barcode:
GCCCACCAAGGAACCATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aftph (216549)
Gene Description:
aftiphilin
Transcript:
RefSeq NM_181411.2 (NON-CURRENT)
Match location:
Position 2482 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCACCAAGGAACCATTAAA
Hairpin Sequence:
5'-CCGG-GCCCACCAAGGAACCATTAAA-CTCGAG-TTTAATGGTTCCTTGGTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCACCAAGGAACCATTAAACTCGAGTTTAATGGTTCCTTGGTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCACCAAGGAACCATTAAACTCGAGTTTAATGGTTCCTTGGTGGGC-3'

Other clones with same target sequence:

(none)