Construct: shRNA TRCN0000277390

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145604.2-400s21c1
DNA Barcode:
CCTCATCCTTAACCTGACTCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
D230025D16Rik (234678)
Gene Description:
RIKEN cDNA D230025D16 gene
Transcript:
RefSeq NM_145604.2 (CURRENT)
Match location:
Position 400 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277390)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATCCTTAACCTGACTCA
Hairpin Sequence:
5'-CCGG-CCTCATCCTTAACCTGACTCA-CTCGAG-TGAGTCAGGTTAAGGATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATCCTTAACCTGACTCACTCGAGTGAGTCAGGTTAAGGATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATCCTTAACCTGACTCACTCGAGTGAGTCAGGTTAAGGATGAGG-3'

Other clones with same target sequence:

  1. TRCN0000182875