Construct: shRNA TRCN0000277415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199149.3-2087s21c1
DNA Barcode:
TCCCACTGAGCAGCGTATTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp1r37 (232947)
Gene Description:
protein phosphatase 1, regulatory subunit 37
Transcript:
RefSeq NM_199149.3 (CURRENT)
Match location:
Position 2087 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCACTGAGCAGCGTATTTC
Hairpin Sequence:
5'-CCGG-TCCCACTGAGCAGCGTATTTC-CTCGAG-GAAATACGCTGCTCAGTGGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCACTGAGCAGCGTATTTCCTCGAGGAAATACGCTGCTCAGTGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCACTGAGCAGCGTATTTCCTCGAGGAAATACGCTGCTCAGTGGGA-3'

Other clones with same target sequence:

(none)