Construct: shRNA TRCN0000277462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016915.3-2428s21c1
DNA Barcode:
CTCCATGTCCATCTGACACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pla2g6 (53357)
Gene Description:
phospholipase A2, group VI
Transcript:
RefSeq NM_016915.3 (NON-CURRENT)
Match location:
Position 2428 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCATGTCCATCTGACACTA
Hairpin Sequence:
5'-CCGG-CTCCATGTCCATCTGACACTA-CTCGAG-TAGTGTCAGATGGACATGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCATGTCCATCTGACACTACTCGAGTAGTGTCAGATGGACATGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCATGTCCATCTGACACTACTCGAGTAGTGTCAGATGGACATGGAG-3'

Other clones with same target sequence:

  1. TRCN0000182230