Construct: shRNA TRCN0000277508

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172276.3-602s21c1
DNA Barcode:
ACTTACGGCAGCGACTATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sfswap (231769)
Gene Description:
splicing factor, suppressor of white-apricot homolog (Drosophila)
Transcript:
RefSeq NM_172276.3 (CURRENT)
Match location:
Position 602 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277508)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTACGGCAGCGACTATTAT
Hairpin Sequence:
5'-CCGG-ACTTACGGCAGCGACTATTAT-CTCGAG-ATAATAGTCGCTGCCGTAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTACGGCAGCGACTATTATCTCGAGATAATAGTCGCTGCCGTAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTACGGCAGCGACTATTATCTCGAGATAATAGTCGCTGCCGTAAGT-3'

Other clones with same target sequence:

(none)