Construct: shRNA TRCN0000277672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153029.3-3191s21c1
DNA Barcode:
GCAGTTACTCTTGAGTGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
N4BP1 (9683)
Gene Description:
NEDD4 binding protein 1
Transcript:
RefSeq NM_153029.3 (NON-CURRENT)
Match location:
Position 3191 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTACTCTTGAGTGTATT
Hairpin Sequence:
5'-CCGG-GCAGTTACTCTTGAGTGTATT-CTCGAG-AATACACTCAAGAGTAACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTACTCTTGAGTGTATTCTCGAGAATACACTCAAGAGTAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTACTCTTGAGTGTATTCTCGAGAATACACTCAAGAGTAACTGC-3'

Other clones with same target sequence:

  1. TRCN0000128563