Construct: shRNA TRCN0000277719

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003334.3-3169s21c1
DNA Barcode:
GCACAAATTAGAGATCACCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBA1 (7317)
Gene Description:
ubiquitin like modifier activating enzyme 1
Transcript:
RefSeq NM_003334.3 (NON-CURRENT)
Match location:
Position 3169 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277719)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAAATTAGAGATCACCAT
Hairpin Sequence:
5'-CCGG-GCACAAATTAGAGATCACCAT-CTCGAG-ATGGTGATCTCTAATTTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAAATTAGAGATCACCATCTCGAGATGGTGATCTCTAATTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAAATTAGAGATCACCATCTCGAGATGGTGATCTCTAATTTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000004002