Construct: shRNA TRCN0000277743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018434.4-687s21c1
DNA Barcode:
CGGTTCTTTGTCCCTCCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF130 (55819)
Gene Description:
ring finger protein 130
Transcript:
RefSeq NM_018434.4 (NON-CURRENT)
Match location:
Position 687 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTCTTTGTCCCTCCTAAT
Hairpin Sequence:
5'-CCGG-CGGTTCTTTGTCCCTCCTAAT-CTCGAG-ATTAGGAGGGACAAAGAACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTCTTTGTCCCTCCTAATCTCGAGATTAGGAGGGACAAAGAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTCTTTGTCCCTCCTAATCTCGAGATTAGGAGGGACAAAGAACCG-3'

Other clones with same target sequence:

  1. TRCN0000007730