Construct: shRNA TRCN0000277755

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015343.4-986s21c1
DNA Barcode:
CCAGCATTGTGATCCTGGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CTDNEP1 (23399)
Gene Description:
CTD nuclear envelope phosphatase 1
Transcript:
RefSeq NM_015343.4 (NON-CURRENT)
Match location:
Position 986 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277755)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCATTGTGATCCTGGATA
Hairpin Sequence:
5'-CCGG-CCAGCATTGTGATCCTGGATA-CTCGAG-TATCCAGGATCACAATGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCATTGTGATCCTGGATACTCGAGTATCCAGGATCACAATGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCATTGTGATCCTGGATACTCGAGTATCCAGGATCACAATGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000006913