Construct: shRNA TRCN0000277788

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024117.3-1597s21c1
DNA Barcode:
CTAAGCAATCACGACTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPKAP1 (79109)
Gene Description:
MAPK associated protein 1
Transcript:
RefSeq NM_024117.3 (CURRENT)
Match location:
Position 1597 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277788)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAAGCAATCACGACTATAAA
Hairpin Sequence:
5'-CCGG-CTAAGCAATCACGACTATAAA-CTCGAG-TTTATAGTCGTGATTGCTTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAAGCAATCACGACTATAAACTCGAGTTTATAGTCGTGATTGCTTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAAGCAATCACGACTATAAACTCGAGTTTATAGTCGTGATTGCTTAG-3'

Other clones with same target sequence:

  1. TRCN0000003151