Construct: shRNA TRCN0000277865

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006742.2-1271s21c1
DNA Barcode:
GCACCGCTCCATATCCCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSKH1 (5681)
Gene Description:
protein serine kinase H1
Transcript:
RefSeq NM_006742.2 (NON-CURRENT)
Match location:
Position 1271 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277865)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCGCTCCATATCCCAGAA
Hairpin Sequence:
5'-CCGG-GCACCGCTCCATATCCCAGAA-CTCGAG-TTCTGGGATATGGAGCGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCGCTCCATATCCCAGAACTCGAGTTCTGGGATATGGAGCGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCGCTCCATATCCCAGAACTCGAGTTCTGGGATATGGAGCGGTGC-3'

Other clones with same target sequence:

  1. TRCN0000001712