Construct: shRNA TRCN0000277887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002803.2-533s21c1
DNA Barcode:
CCTAAGATTGACCCAACAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSMC2 (5701)
Gene Description:
proteasome 26S subunit, ATPase 2
Transcript:
RefSeq NM_002803.2 (NON-CURRENT)
Match location:
Position 533 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000277887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAGATTGACCCAACAGTT
Hairpin Sequence:
5'-CCGG-CCTAAGATTGACCCAACAGTT-CTCGAG-AACTGTTGGGTCAATCTTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAGATTGACCCAACAGTTCTCGAGAACTGTTGGGTCAATCTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAGATTGACCCAACAGTTCTCGAGAACTGTTGGGTCAATCTTAGG-3'

Other clones with same target sequence:

  1. TRCN0000007185