Construct: shRNA TRCN0000278355

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020992.2-669s21c1
DNA Barcode:
GCCTTGTCATCGACAAAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDLIM1 (9124)
Gene Description:
PDZ and LIM domain 1
Transcript:
RefSeq NM_020992.2 (NON-CURRENT)
Match location:
Position 669 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278355)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGTCATCGACAAAGAAT
Hairpin Sequence:
5'-CCGG-GCCTTGTCATCGACAAAGAAT-CTCGAG-ATTCTTTGTCGATGACAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGTCATCGACAAAGAATCTCGAGATTCTTTGTCGATGACAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGTCATCGACAAAGAATCTCGAGATTCTTTGTCGATGACAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000161426