Construct: shRNA TRCN0000278466

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024090.2-1287s21c1
DNA Barcode:
CCAGTAAGTTTATGATCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELOVL6 (79071)
Gene Description:
ELOVL fatty acid elongase 6
Transcript:
RefSeq NM_024090.2 (NON-CURRENT)
Match location:
Position 1287 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278466)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTAAGTTTATGATCCTTT
Hairpin Sequence:
5'-CCGG-CCAGTAAGTTTATGATCCTTT-CTCGAG-AAAGGATCATAAACTTACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTAAGTTTATGATCCTTTCTCGAGAAAGGATCATAAACTTACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTAAGTTTATGATCCTTTCTCGAGAAAGGATCATAAACTTACTGG-3'

Other clones with same target sequence:

  1. TRCN0000160240