Construct: shRNA TRCN0000278490

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002431.2-448s21c1
DNA Barcode:
GTGGATTTGGACAACACCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MNAT1 (4331)
Gene Description:
MNAT1 component of CDK activating kinase
Transcript:
RefSeq NM_002431.2 (NON-CURRENT)
Match location:
Position 448 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278490)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGATTTGGACAACACCAAA
Hairpin Sequence:
5'-CCGG-GTGGATTTGGACAACACCAAA-CTCGAG-TTTGGTGTTGTCCAAATCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGATTTGGACAACACCAAACTCGAGTTTGGTGTTGTCCAAATCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGATTTGGACAACACCAAACTCGAGTTTGGTGTTGTCCAAATCCAC-3'

Other clones with same target sequence:

  1. TRCN0000019946