Construct: shRNA TRCN0000278521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004855.4-403s21c1
DNA Barcode:
CGGCAAGATAAAGCTGCGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIGB (9488)
Gene Description:
phosphatidylinositol glycan anchor biosynthesis class B
Transcript:
RefSeq NM_004855.4 (NON-CURRENT)
Match location:
Position 403 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAAGATAAAGCTGCGAAA
Hairpin Sequence:
5'-CCGG-CGGCAAGATAAAGCTGCGAAA-CTCGAG-TTTCGCAGCTTTATCTTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAAGATAAAGCTGCGAAACTCGAGTTTCGCAGCTTTATCTTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAAGATAAAGCTGCGAAACTCGAGTTTCGCAGCTTTATCTTGCCG-3'

Other clones with same target sequence:

  1. TRCN0000157804