Construct: shRNA TRCN0000278524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030979.2-1097s21c1
DNA Barcode:
GCCACTAAAGCAGTTACAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PABPC3 (5042)
Gene Description:
poly(A) binding protein cytoplasmic 3
Transcript:
RefSeq NM_030979.2 (CURRENT)
Match location:
Position 1097 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACTAAAGCAGTTACAGAA
Hairpin Sequence:
5'-CCGG-GCCACTAAAGCAGTTACAGAA-CTCGAG-TTCTGTAACTGCTTTAGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACTAAAGCAGTTACAGAACTCGAGTTCTGTAACTGCTTTAGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACTAAAGCAGTTACAGAACTCGAGTTCTGTAACTGCTTTAGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000161683