Construct: shRNA TRCN0000278570

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003829.3-5490s21c1
DNA Barcode:
GTCACTGGAAAGTAGCTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MPDZ (8777)
Gene Description:
multiple PDZ domain crumbs cell polarity complex component
Transcript:
RefSeq NM_003829.3 (NON-CURRENT)
Match location:
Position 5490 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278570)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCACTGGAAAGTAGCTCAAA
Hairpin Sequence:
5'-CCGG-GTCACTGGAAAGTAGCTCAAA-CTCGAG-TTTGAGCTACTTTCCAGTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCACTGGAAAGTAGCTCAAACTCGAGTTTGAGCTACTTTCCAGTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCACTGGAAAGTAGCTCAAACTCGAGTTTGAGCTACTTTCCAGTGAC-3'

Other clones with same target sequence:

  1. TRCN0000141979