Construct: shRNA TRCN0000278582

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015686.2-485s21c1
DNA Barcode:
CTTGCACCAAATTGCAATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM155B (27112)
Gene Description:
family with sequence similarity 155 member B
Transcript:
RefSeq NM_015686.2 (CURRENT)
Match location:
Position 485 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278582)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGCACCAAATTGCAATCTT
Hairpin Sequence:
5'-CCGG-CTTGCACCAAATTGCAATCTT-CTCGAG-AAGATTGCAATTTGGTGCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGCACCAAATTGCAATCTTCTCGAGAAGATTGCAATTTGGTGCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGCACCAAATTGCAATCTTCTCGAGAAGATTGCAATTTGGTGCAAG-3'

Other clones with same target sequence:

  1. TRCN0000160442