Construct: shRNA TRCN0000278672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013881.3-1045s21c1
DNA Barcode:
CCAAAGACTCTGCGAGTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ulk2 (29869)
Gene Description:
unc-51 like kinase 2
Transcript:
RefSeq NM_013881.3 (NON-CURRENT)
Match location:
Position 1045 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGACTCTGCGAGTAATA
Hairpin Sequence:
5'-CCGG-CCAAAGACTCTGCGAGTAATA-CTCGAG-TATTACTCGCAGAGTCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGACTCTGCGAGTAATACTCGAGTATTACTCGCAGAGTCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGACTCTGCGAGTAATACTCGAGTATTACTCGCAGAGTCTTTGG-3'

Other clones with same target sequence:

  1. TRCN0000222265