Construct: shRNA TRCN0000278682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138306.1-2124s21c1
DNA Barcode:
CGAGGCTCTACATTATGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dgkz (104418)
Gene Description:
diacylglycerol kinase zeta
Transcript:
RefSeq NM_138306.1 (NON-CURRENT)
Match location:
Position 2124 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGCTCTACATTATGACAA
Hairpin Sequence:
5'-CCGG-CGAGGCTCTACATTATGACAA-CTCGAG-TTGTCATAATGTAGAGCCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGCTCTACATTATGACAACTCGAGTTGTCATAATGTAGAGCCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGCTCTACATTATGACAACTCGAGTTGTCATAATGTAGAGCCTCG-3'

Other clones with same target sequence:

  1. TRCN0000025397