Construct: shRNA TRCN0000278731

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007509.2-1119s21c1
DNA Barcode:
CTGACTTGACTGGGTACATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp6v1b2 (11966)
Gene Description:
ATPase, H+ transporting, lysosomal V1 subunit B2
Transcript:
RefSeq NM_007509.2 (NON-CURRENT)
Match location:
Position 1119 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278731)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGACTTGACTGGGTACATTA
Hairpin Sequence:
5'-CCGG-CTGACTTGACTGGGTACATTA-CTCGAG-TAATGTACCCAGTCAAGTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGACTTGACTGGGTACATTACTCGAGTAATGTACCCAGTCAAGTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGACTTGACTGGGTACATTACTCGAGTAATGTACCCAGTCAAGTCAG-3'

Other clones with same target sequence:

  1. TRCN0000198772