Construct: shRNA TRCN0000278786

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028233.2-2458s21c1
DNA Barcode:
GCGTGGAACTTGAAACAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrpprc (72416)
Gene Description:
leucine-rich PPR-motif containing
Transcript:
RefSeq NM_028233.2 (CURRENT)
Match location:
Position 2458 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278786)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGGAACTTGAAACAAGAA
Hairpin Sequence:
5'-CCGG-GCGTGGAACTTGAAACAAGAA-CTCGAG-TTCTTGTTTCAAGTTCCACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGGAACTTGAAACAAGAACTCGAGTTCTTGTTTCAAGTTCCACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGGAACTTGAAACAAGAACTCGAGTTCTTGTTTCAAGTTCCACGC-3'

Other clones with same target sequence:

  1. TRCN0000173491