Construct: shRNA TRCN0000278815

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021897.3-857s21c1
DNA Barcode:
GTCTTTGAACAGAAATGGTCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trp53inp1 (60599)
Gene Description:
transformation related protein 53 inducible nuclear protein 1
Transcript:
RefSeq NM_021897.3 (CURRENT)
Match location:
Position 857 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278815)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTTTGAACAGAAATGGTCT
Hairpin Sequence:
5'-CCGG-GTCTTTGAACAGAAATGGTCT-CTCGAG-AGACCATTTCTGTTCAAAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTTTGAACAGAAATGGTCTCTCGAGAGACCATTTCTGTTCAAAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTTTGAACAGAAATGGTCTCTCGAGAGACCATTTCTGTTCAAAGAC-3'

Other clones with same target sequence:

  1. TRCN0000174801