Construct: shRNA TRCN0000278858

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014679.3-339s21c1
DNA Barcode:
CCTTCGGATAAGCCTTTCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CEP57 (9702)
Gene Description:
centrosomal protein 57
Transcript:
RefSeq NM_014679.3 (NON-CURRENT)
Match location:
Position 339 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278858)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCGGATAAGCCTTTCCTT
Hairpin Sequence:
5'-CCGG-CCTTCGGATAAGCCTTTCCTT-CTCGAG-AAGGAAAGGCTTATCCGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCGGATAAGCCTTTCCTTCTCGAGAAGGAAAGGCTTATCCGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCGGATAAGCCTTTCCTTCTCGAGAAGGAAAGGCTTATCCGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000141011