Construct: shRNA TRCN0000278904

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021067.3-328s21c1
DNA Barcode:
GACACTGTTCTCTGTTAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GINS1 (9837)
Gene Description:
GINS complex subunit 1
Transcript:
RefSeq NM_021067.3 (NON-CURRENT)
Match location:
Position 328 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278904)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACACTGTTCTCTGTTAAGAA
Hairpin Sequence:
5'-CCGG-GACACTGTTCTCTGTTAAGAA-CTCGAG-TTCTTAACAGAGAACAGTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACACTGTTCTCTGTTAAGAACTCGAGTTCTTAACAGAGAACAGTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACACTGTTCTCTGTTAAGAACTCGAGTTCTTAACAGAGAACAGTGTC-3'

Other clones with same target sequence:

  1. TRCN0000128417