Construct: shRNA TRCN0000278966

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024585.2-1690s21c1
DNA Barcode:
CCTCTGCTGTCACTCAATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARMC7 (79637)
Gene Description:
armadillo repeat containing 7
Transcript:
RefSeq NM_024585.2 (NON-CURRENT)
Match location:
Position 1690 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000278966)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGCTGTCACTCAATGAT
Hairpin Sequence:
5'-CCGG-CCTCTGCTGTCACTCAATGAT-CTCGAG-ATCATTGAGTGACAGCAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGCTGTCACTCAATGATCTCGAGATCATTGAGTGACAGCAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGCTGTCACTCAATGATCTCGAGATCATTGAGTGACAGCAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000139716