Construct: shRNA TRCN0000279004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023422.3-463s21c1
DNA Barcode:
CTGGATGCTGTAACCTCAAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist1h2bc (68024)
Gene Description:
histone cluster 1, H2bc
Transcript:
RefSeq NM_023422.3 (CURRENT)
Match location:
Position 463 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGATGCTGTAACCTCAAGA
Hairpin Sequence:
5'-CCGG-CTGGATGCTGTAACCTCAAGA-CTCGAG-TCTTGAGGTTACAGCATCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGATGCTGTAACCTCAAGACTCGAGTCTTGAGGTTACAGCATCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGATGCTGTAACCTCAAGACTCGAGTCTTGAGGTTACAGCATCCAG-3'

Other clones with same target sequence:

(none)