Construct: shRNA TRCN0000279019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177282.4-3239s21c1
DNA Barcode:
GCCAGCATCTGTAACCAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mical2 (320878)
Gene Description:
microtubule associated monooxygenase, calponin and LIM domain containing 2
Transcript:
RefSeq NM_177282.4 (NON-CURRENT)
Match location:
Position 3239 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGCATCTGTAACCAATTA
Hairpin Sequence:
5'-CCGG-GCCAGCATCTGTAACCAATTA-CTCGAG-TAATTGGTTACAGATGCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGCATCTGTAACCAATTACTCGAGTAATTGGTTACAGATGCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGCATCTGTAACCAATTACTCGAGTAATTGGTTACAGATGCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000192634